Journal: The Journal of Clinical Investigation
Article Title: Staphylococcus aureus accessory gene regulator quorum-sensing system inhibits keratinocyte lipid enzymes and delays wound repair
doi: 10.1172/JCI190411
Figure Lengend Snippet: ( A ) Representative images of murine wounds 7 days after infection with indicated S . aureus mutants. ( B ) Bacteria recovered from day 7 murine wounds infected with S . aureus mutants. ( C ) Quantification of closure on day 7 of murine wounds infected with S . aureus mutants. ( D ) LDH release from scratch-wounded primary NHEKs 24 hours after treatment with CM of S . aureus mutants (3 × 10 7 CFU/mL equivalent). ( E ) Scratch-wounded NHEK migration after 24-hour treatment with CM of S . aureus mutants (3 × 10 7 CFU/mL equivalent). ( F ) Proliferation measured with MTT assay of NHEKs after 24-hour treatment with CM of S . aureus mutants (3 × 10 7 CFU/mL equivalent). ( G ) KI67 gene expression in proliferating NHEKs treated for 24 hours with CM of S . aureus mutants (3 × 10 7 CFU/mL equivalent). One-way ANOVA followed by Bonferroni’s multiple-comparison adjustment for more than 2 groups ( B – G ). Experiments were performed at least twice. Data represent mean ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.
Article Snippet: Primary NHEKs (ATCC, PCS-200-010) were cultured in EpiLife medium containing 60 μM CaCl 2 (Gibco, MEPI500CA) supplemented with Human Keratinocyte Growth Supplement (Gibco, S0015) and antibiotic-antimycotic (penicillin 100 U/mL, streptomycin 100 U/mL, and amphotericin B 250 ng/mL; Gibco, 15240062) at 37°C, 5% CO 2 .
Techniques: Infection, Bacteria, Migration, MTT Assay, Gene Expression, Comparison
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